2 edition of nature and synthesis of mitochondrial RNA during early Xenopus laevis development. found in the catalog.
nature and synthesis of mitochondrial RNA during early Xenopus laevis development.
Paul Gary Young
Written in English
|The Physical Object|
|Pagination||ix, 132 leaves.|
|Number of Pages||132|
We have set up a heterologous in vivo system to study gene regulation at the post-transcriptional level during early development. This system uses two amphibian species, Xenopus laevis and. Mitochondrial differentiation during the early development of the amphibian embryo. Lennart Nelson. Mitochondria from Xenopus laevis and Ambystoma mexica- num embryos between fertilization and the beginning of feeding were studied: the former with respect to metabo lic behaviour, enzyme pattern and carrier activity, and.
In this study, we used hybridization techniques with cloned sequences to measure the titers of 23 mitochondrial and non-mitochondrial polyadenylate-containing [poly(A)+]RNA species during early development in the frog Xenopus laevis. These RNA species were some of the most abundant cellular poly(A)+ RNA species in early by: Xenopus laevis is an ideal system to study asymmetric cell division during early development, because of the huge size of its oocytes and early-stage blastomeres. Here, we present the current knowledge about localization and distribution of cell fate determinants along the three body axes: animal–vegetal, dorsal–ventral, and left–: Radek Sindelka, Monika Sidova, Pavel Abaffy, Mikael Kubista.
In Xenopus laevis the mitochondrial cloud increases during previtellogenesis (Callen et al., ; Mignotte et al., ) and the cloud contains many intermingled mitochondria and small vesicles. RNA metabolism during amphibian oogenesis has been investigated by the analysis of individual cDNA cloned sequences. Two cDNA clone libraries were constructed from the pA+ RNA of Xenopus ovary and of tadpole. The accumulation of RNAs complementary to individual clones was examined during oogenesis by isolating RNA from oocytes of different by:
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A study on the steady-state population of poly(A)+RNA during early development of Xenopus laevis N. Sagata, K. Shiokawa & K. Yamana; Developmental Biology ()Cited by: J.
Mol. Bid. () 63, Mitochondrial RNA m Xenopus laevis I. ThExpression of the Mitochondrial Genome IGOE B. DAWID Department of Embryology Carnegie Institution of Washington Baltimore, Md.U.S.A.
(Beceived 26 May ) The bulk of RNA in mitochondria isolated from eggs and ovaries of Xenoptts laevis is represented by the large and small mitochondrial rRNAf and Cited by: The RNA of the mature oocyte of Xenopus laevis has been studied in many laboratories and more extensively characterized than that of any other oocyte.
Some of the information which has been developed relative to this RNA is summarized briefly in the paragraphs by: 1. Mol. Biol. () 63, Mitochondrial RNA in Xenopus laevis Molecular Weights and Other Physical Properties of Mitochondrial Ribosomal and 4 s RNA IGOE B.
DAWID ATO JOHN W. CHASE Department of Embryology Carnegie Institution of Washington Baltimore, Md. U.S.A. (Beceived 26 May } The nucleotide composition and physical properties of the large and small mitochondrial Cited by: During the early development of Xenopus laevis, we followed in individual nuclei the formation of a nucleolus by examining simultaneously its structural organization and its transcriptional.
Embryonic cells of Xenopus laevis were labeled for varying lengths of time, and their nuclear and cytoplasmic RNAs were analyzed, with the following results. (1) The synthesis of small nuclear RNAs (snRNAs) is detected from blastula stage on.
(2) The initiation of 4 S and 5 S RNA Cited by: No incorporation of label into ribosomal RNA is seen in pregastrula stages be it whole cell or ribosome extracts.
These data support the observation that during early embryogenesis of Xenopus the synthesis of ribosomal RNA is under regulation. The stage-specific pattern of ribosomal RNA Cited by: 3.
Finally, the acquisition of the heat-shock response (i.e., synthesis of hsp and accumulation of hsp70 mRNA) during early Xenopus development is correlated with the acquisition of thermotolerance.
In hybrid inviability between Xenopus laevis and Xenopus tropicalis, genomic regions on two X. laevis chromosomes are incompatible with the X. tropicalis cytoplasm and are mis-segregated during Cited by: Early Development of Xenopus laevis: A Laboratory Manual This manual contains a comprehensive collection of protocols for the study of early development in Xenopus embryos.
It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught. Genome evolution in the allotetraploid frog Xenopus laevis The two homoeologous subgenomes in the allotetraploid frog Xenopus laevis evolved asymmetrically; one often retained the ancestral state, whereas the other experienced gene loss, deletion, rearrangement and.
Abstract. Asymmetric distribution of fate determinants within cells is an essential biological strategy to prepare them for asymmetric division.
In this work we measure the intracellular distribution of 12 maternal microRNAs (miRNA) along the animal-vegetal axis of the Xenopus laevis oocyte using qPCR by: 7. Regular Article. rDNA Transcription during Xenopus laevis Oogenesis. Abstract. It is generally believed that, during Xenopus laevis oogenesis, polymerase I transcription is high in the early vitellogenic oocytes (stages III and IV) and very low in later by: 5.
Transposable elements in the genome are generally silenced in differentiated somatic cells. However, increasing evidence indicates that some of them are actively transcribed in early embryos and the proper regulation of retrotransposon expression is essential for normal by: 7.
Mitochondrial transcription in the earlyXenopus laevis embryo resumes several hours before active mtDNA replication, effectively decoupling mtDNA transcription and replication.
This developmental feature makes Xenopusembryogenesis an appealing model system to investigate the regulation of mitochondrial transcription.
Mitochondrial gene expression has been analysed during embryonic development of Xenopus laevis; the relative amounts of 12S and 16S ribosomal RNAs and of most mitochondrial messenger RNAs were determined by slot-blot and Northern-blot hybridization experiments with specific mitochondrial DNA by: Abstract.
Mitochondrial gene expression has been analysed during embryonic development of Xenopus laevis; the relative amounts of 12S and 16S ribosomal RNAs and of most mitochondrial messenger RNAs were determined by slot-blot and Northern-blot hybridization experiments with specific mitochondrial DNA probes.
Gene expression in Xenopus embryogenesis more importantly, in its complexity. There are s differen+ t poly(A) RNA species in the egg (Davidson & HoughPerlma; n & Rosbash, ), a value that is on the high side of the range of RNA.
Abstract. Studies examining the messenger RNA population present in Xenopus laevis oocytes and early embryos are discussed. There may be two classes of mRNA present in the oocyte, one which is used by the developing germ cell and the other which is synthesized during early oogenesis and stored for translation after by: 5.
We show that RNA-bearing late endosomes often pause on mitochondria and that mRNAs encoding proteins for mitochondrial function are translated on Rab7a endosomes.
Disruption of Rab7a function with Rab7a mutants, including those associated with Charcot-Marie-Tooth type 2B neuropathy, markedly decreases axonal protein synthesis, impairs Cited by:. The regulation of ribosome production is an important aspect of gene expression during Xenopus laevis development.
K., and Yamana, K.,A study on the steady-state population of poly(A) + RNA during early development of Xenopus laevis, Dev. Biol. J. R.,In the absence of ribosomal RNA synthesis, the ribosomal proteins Cited by: 4.
The role of the mitochondrial genome in early development and differentiation was studied in mouse embryos cultured in vitro from the two to four cell stage to the blastocyst (about cells).
During this period the mitochondria undergo morphological differentiation: progressive enlargement followed by an increase in matrix density, in number of cristae, and in number of mitochondrial Cited by: Cytoplasmic control of nuclear DNA synthesis during early development of Xenopus laevis: a cell-free assay.
This article has been cited by other articles in PMC. Abstract. Nuclei isolated from nondividing cells were induced to synthesize DNA by incubation with cytoplasm from early embryos of Cited by: